nuclear staining with dapi (Beyotime)
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nuclear staining with dapi
Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+nuclear+staining/DAPI/pm42791620-89-34-39
Average 99 stars, based on 33153 article reviews
Nuclear Staining With Dapi, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 33153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+nuclear+staining/DAPI/pm42791620-89-34-39
Average 99 stars, based on 33153 article reviews
nuclear staining with dapi - by Bioz Stars,
2026-11
99/100 stars
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Incubation:Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus. Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by Article Title: Hydrogen gas inhibits neuroinflammation after traumatic brain injury by promoting microglia M2 polarization via AMPK-dependent mediation of HDAC5 Article Snippet: .. Samples were incubated with the following primary antibodies: mouse monoclonal anti-iNOS (Abcam, Cambridge, UK, # ab49999, 1:50) or rabbit monoclonal anti-TREM-2 (Abcam, # ab245227, 1:100) at 4°C overnight, washed three times with PBS, and then incubated with Cy3-conjugated (Proteintech, Rosemont, IL, USA, #SA00009-2, 1:200) or Page 11/29 uorescein (FITC)-conjugated (Proteintech, #SA00003-1, 1:200) secondary antibodies at 37°C for 1 h. After additional PBS washes, Staining:Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus. Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by Article Title: Development of a Colloidal Gold-Based Immunochromatographic Strip Targeting the Nucleoprotein for Rapid Detection of Canine Distemper Virus Article Snippet: .. Cells were then incubated with FITC-conjugated goat anti-mouse IgG (1:1000; Cat. No.: A-11001; Thermo Fisher, USA), followed by Article Title: Novel genetic insights into causal effects of potential metformin targets and immune mediation on sepsis. Article Snippet: .. After five rounds of primary and secondary antibodies incubations, Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Article Title: Knockdown of CHI3L1 Attenuates Venous Intimal Hyperplasia by Modulating Vascular Inflammation and Oxidative Stress via the JNK/ERK Pathway Article Snippet: They were then incubated with primary antibodies (α-SMA: 1:400, 67735-1- Ig, Proteintech; CHI3L1: 1:100, PA5-95897, Invitrogen) overnight at 4 °C, followed by fluorescent secondary antibodies (1:500, SA00013-2; 1:200, SA00013-3, Proteintech) for 1 h in the dark. .. After Article Title: Hla of Staphylococcus aureus suppresses TRAF6 K63-linked ubiquitination to promote intracellular persistence Article Snippet: For confocal microscopy, BMDMs were fixed with 231 Jo urn al Pr e-p roo f 12 immunostaining fixative solution (Beyotime, P0098) for 20 min at room temperature, 232 permeabilized with 0.5% Triton X-100 (Beyotime, P0096) for 30 min, and blocked with 233 immunostaining blocking solution (Beyotime, P0102) for 1 h. Cells were incubated 234 overnight at 4 °C with primary antibodies, followed by incubation with secondary 235 antibodies for 1 h at room temperature. .. After Article Title: Inhibition of neutrophil infiltration and NETosis ameliorates sepsis-induced cardiac injury and reduces myocardial inflammation and apoptosis. Article Snippet: Aims: Sepsis-induced cardiomyopathy (SIC) lacks effective targeted therapies.. This study investigated whether inhibiting neutrophil infiltration and neutrophil extracellular traps (NETosis) preserves cardiac function and mitigates inflammation and apoptosis in sepsis.. Methods: We performed analyses of gene-expression microarray datasets and single-cell RNA sequencing to define the SIC landscape, and generated cardiac RNA sequencing in LPS-treated versus control mice to validate microarray signatures. Article Title: Hydrogen gas inhibits neuroinflammation after traumatic brain injury by promoting microglia M2 polarization via AMPK-dependent mediation of HDAC5 Article Snippet: .. Samples were incubated with the following primary antibodies: mouse monoclonal anti-iNOS (Abcam, Cambridge, UK, # ab49999, 1:50) or rabbit monoclonal anti-TREM-2 (Abcam, # ab245227, 1:100) at 4°C overnight, washed three times with PBS, and then incubated with Cy3-conjugated (Proteintech, Rosemont, IL, USA, #SA00009-2, 1:200) or Page 11/29 uorescein (FITC)-conjugated (Proteintech, #SA00003-1, 1:200) secondary antibodies at 37°C for 1 h. After additional PBS washes, TUNEL Assay:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Article Title: Inhibition of neutrophil infiltration and NETosis ameliorates sepsis-induced cardiac injury and reduces myocardial inflammation and apoptosis. Article Snippet: Aims: Sepsis-induced cardiomyopathy (SIC) lacks effective targeted therapies.. This study investigated whether inhibiting neutrophil infiltration and neutrophil extracellular traps (NETosis) preserves cardiac function and mitigates inflammation and apoptosis in sepsis.. Methods: We performed analyses of gene-expression microarray datasets and single-cell RNA sequencing to define the SIC landscape, and generated cardiac RNA sequencing in LPS-treated versus control mice to validate microarray signatures. Luciferase:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Reporter Gene Assay:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. cDNA Synthesis:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Lysis:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Western Blot:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. CCK-8 Assay:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Bicinchoninic Acid Protein Assay:Article Title: Cytopathic bovine viral diarrhea virus-induced NLRP3 inflammasome activation triggers GSDMD-mediated pyroptosis, amplifying the inflammatory response. Article Snippet: Bovine viral diarrhea virus (BVDV) is the causative agent of bovine viral diarrhea-mucosal disease, which is characterized by diarrhea, enteritis, and erosion and necrosis of intestinal mucosa, causing substantial economic losses in the cattle industry.. Previous research has shown that cytopathic BVDV (cpBVDV) can activate the nucleotide oligomerization domain (NOD)-like receptor protein 3 (NLRP3) inflammasome, whereas noncytopathic BVDV (ncpBVDV) cannot.. Nonetheless, the role of the NLRP3 inflammasome in inflammatory responses triggered by cpBVDV remains poorly understood. Confocal:Article Title: Hla of Staphylococcus aureus suppresses TRAF6 K63-linked ubiquitination to promote intracellular persistence Article Snippet: For confocal microscopy, BMDMs were fixed with 231 Jo urn al Pr e-p roo f 12 immunostaining fixative solution (Beyotime, P0098) for 20 min at room temperature, 232 permeabilized with 0.5% Triton X-100 (Beyotime, P0096) for 30 min, and blocked with 233 immunostaining blocking solution (Beyotime, P0102) for 1 h. Cells were incubated 234 overnight at 4 °C with primary antibodies, followed by incubation with secondary 235 antibodies for 1 h at room temperature. .. After Laser-Scanning Microscopy:Article Title: Hla of Staphylococcus aureus suppresses TRAF6 K63-linked ubiquitination to promote intracellular persistence Article Snippet: For confocal microscopy, BMDMs were fixed with 231 Jo urn al Pr e-p roo f 12 immunostaining fixative solution (Beyotime, P0098) for 20 min at room temperature, 232 permeabilized with 0.5% Triton X-100 (Beyotime, P0096) for 30 min, and blocked with 233 immunostaining blocking solution (Beyotime, P0102) for 1 h. Cells were incubated 234 overnight at 4 °C with primary antibodies, followed by incubation with secondary 235 antibodies for 1 h at room temperature. .. After |